Suppression of synaptic activity was achieved by changing cultured cells into serum-free base-medium (10% minimum essential media (MEM, Life Technologies) and 90% saltglucoseglycine (SGG) containing in mM: 114 NaCl, NaHCO3, 5.292 KCl, 1 MgCl2, 2 CaCl2, 10 HEPES, 1 Glycine, 30 Glucose, 0.5 sodium pyruvate, 0.1% Phenol Red) with 100 nM tetradotoxin (TTX, Tocris) on DIV9 for 24 h to inhibit neuronal action potential firing
Physicians advice before use is recommended
(iii) increased production of cytotoxic ROS 155,156
The non-selective receptor activation profile makes Melanotan II a useful tool for studying the broader melanocortin system in published preclinical research
Turco G, Chang C, Wang RY, Kim G, Stoops EH, Richardson B, et al